由於9n年大考出了一題,結果與舊教材及每個學過高中生物的學生所認識不同 我查了好久, 而國內大學對這類簡單的化學分析卻沒有提出答案, 任由考試引導教學內容 也不妥, 至少我看到的報告是 大陸--濾紙效果--胡黃ab 香港--濾紙效果--胡黃ab 美國--高中教材參考--濾紙效果--胡黃ab 其他網頁--薄層分析效果--胡ab黃 只有最後一項與該年大考所示答案一致, 而解釋卻共用了極性問題來概括 困擾了兩年, 在google用不同詞組搜尋, 看到這討論..摘述部份內容 "We do a chromatographic separation that sounds very much like Michael's and I thought the pigment order was chlorophyll b chlorophyll a xanthophyll 2 xanthophyll 1 (gray, "ides") and carotene at the solvent front. ... If run on a silica gel plate, I would expect the following pigments: carotenoids at solvent front, followed by a dull grey band of chlorophyll decomposition products (depending on how old the spinach prep is), followed by chlorophyll a then b then three xanthophylls (one fairly bright with the others fairly dull). ... We also do a separation of spinach in petroleum ether/ acetone on paper. The separation is based on the polarity of the pigments - least polar should migrate farthest. So at/ near the solvent front is carotene followed by xanthophyll, chl a and chl b. This order is the same as most other replies to the list. Every once in a while we get odd results. Check all the components of your set up for contamination. " http://biae.clemson.edu/biolab/spinach.html 在其中似乎也有些argue, 但大概可以見到兩分析方法有不同結果 因此現在都只會教同學記跑最快的胡蘿蔔素, 其他我沒有answer 知道者其實只要將幾個band跑吸光光譜就好了, 當年若是立即有各大學立刻討論,也許不會有此困擾 還好沒影響分數, 不然這帳要向誰算 -- Ξ Origin: 中興大學天樞資訊網 <bbs.nchu.edu.tw> Ξ From : 203.68.192.30