PCR NEWS - July 2011 - focus on circulating microRNAs ----------------------------------------------------------------------- Dear researcher, dear Gene Quantification page reader, Our newsletter informs about the latest news in quantitative real-time PCR (qPCR and qRT-PCR), which are compiled and summarised on the Gene Quantification homepage. The focus of this newsletter issue is: - Update - circulating nucleic acids - focus on microRNA - http://CNA.gene-quantification.info - Recorded qPCR 2011 Event =3D> qPCR 2011 eConference - http://eConference.qPCR2011.net - US qPCR Symposium in Santa Clara, CA - 1st-4th November 2011 - http://EVENTS.gene-quantification.info - MIQE qPCR APP for iPhone, iPad and iPod - iOS Universal - http://MIQE.gene-quantification.info ---------------------------------------------------------------------------= -- Circulating Nucleic Acids (CNA) - Introduction http://CNA.gene-quantification.info Most of the DNA and RNA in the body is located within cells, but a small amount of nucleic acids can also be found circulating freely in the blood. These DNA, RNA and small RNA molecules are thought to come from dying cells that release their contents into the blood as they break down. The term "Circulating Nucleic Acids =3DCNA" refers to segments of DNA or RNA found in the bloodstream. CNAs offers a non-invasive approach to a wide range in diagnostics of clinical disorders that will allow the basic information necessary not only for use in predictive medicine but also for direct use in acute medicine. Further free CNAs offer unique opportunities for early diagnosis of clinical conditions, e.g. in early cancer detection. Although DNA was first demonstrated in human blood from healthy donors, pregnant women and clinical patients in 1948, the structure of DNA was still to be determined as was the elucidation of its role as the basis of the gene. Consequently, no interest was shown in the presence of DNA in the circulatory system until high DNA levels were demonstrated in the blood of patients with systemic lupus erythematosus. Similar observations were also made in acute medicine, diabetes, oncology and fetal medicine. The presence of DNA and RNA in plasma of patients has been recognised since the 1970s - see here =3D> http://CNA.gene-quantification.info ---------------------------------------------------------------------------= -- new CNA microRNA papers: Extracellular microRNA: A new source of biomarkers. Etheridge A, Lee I, Hood L, Galas D, Wang K. Mutat Res. 2011 Mar 23 Institute for Systems Biology, 1441 North 34th Street, Seattle, WA 98103, United States. Circulating microRNAs: Association with disease and potential use as biomarkers. Reid G, Kirschner MB, van Zandwijk N. Critical Reviews in Oncology/Hematology (2010) Asbestos Diseases Research Institute (ADRI), Bernie Banton Centre, University of Sydney, Gate 3, Hospital Road, Concord, Sydney, NSW 2139, Australia. Analysis of circulating microRNA - preanalytical and analytical challenges. McDonald JS, Milosevic D, Reddi HV, Grebe SK, Algeciras-Schimnich A. Clin Chem. 2011 57(6): 833-840 Department of Laboratory Medicine and Pathology, MayoClinic, Rochester, MN 55905, USA. Circulating microRNAs: promising breast cancer biomarkers Helen M Heneghan, Nicola Miller and Michael J Kerin Breast Cancer Research 2011, 13:402 Intracellular and extracellular microRNAs in breast cancer. Corcoran C, Friel AM, Duffy MJ, Crown J, O'Driscoll L. Clin Chem. 2011 57(1): 18-32 School of Pharmacy and Pharmaceutical Sciences and Molecular Therapeutics for Cancer Ireland (MTCI), Trinity College Dublin, Dublin, Ireland. Serum microRNAs as non-invasive biomarkers for cancer. Brase JC, Wuttig D, Kuner R, S=FCltmann H. Mol Cancer. 2010 9: 306. Working Group Cancer Genome Research, German Cancer Research Center, Heidelberg, Germany Cell-free nucleic acids as biomarkers in cancer patients Heidi Schwarzenbach, Dave S. B. Hoon and Klaus Pantel Nat Rev Cancer. 2011 11(6): 426-437 Institute of Tumour Biology, Center of Experimental Medicine, University Medical Center Hamburg-Eppendorf, Hamburg 20246, Germany. Diagnostic applications of cell-free and circulating tumor cell- associated miRNAs in cancer patients. Mostert B, Sieuwerts AM, Martens JW, Sleijfer S. Expert Rev Mol Diagn. 2011 11(3): 259-275 Daniel den Hoed Cancer Center, Laboratory of Clinical Tumor Immunology, Rotterdam, The Netherlands. Identification of muscle-specific microRNAs in serum of muscular dystrophy animal models: promising novel blood-based markers for muscular dystrophy. Mizuno H, Nakamura A, Aoki Y, Ito N, Kishi S, Yamamoto K, Sekiguchi M, Takeda S, Hashido K. PLoS One. 2011 6(3): e18388 Administrative Section of Radiation Protection, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Tokyo, Japan. Serum microRNA signatures identified in a genome-wide serum microRNA expression profiling predict survival of non-small-cell lung cancer. Hu Z, Chen X, Zhao Y, Tian T, Jin G, Shu Y, Chen Y, Xu L, Zen K, Zhang C, Shen H. J Clin Oncol. 2010 28(10): 1721-1726 Department of Epidemiology and Biostatistics, Cancer Center, Nanjing Medical University, 140 Hanzhong Rd, Nanjing 210029, China. more papers on our CNA sub-domain =3D> http://CNA.gene-quantification.info ---------------------------------------------------------------------------= ----- Did you miss the qPCR 2011 Symposium in Freising? BioEPS is now presenting the qPCR 2011 eConference online via video stream! With our new eConference streaming server a world-wide know-how transfer becomes possible in a fast and simple way! qPCR 2011 Event now available NOW as eConfernce =3D> http://eConference.qP= CR2011.net At the event around 500 attendee from 47 contries participated from 28th March - 1th April 2011. During the scientific symposium the presented talks were recorded in excellent sound quality and high movie resolution. The qPCR 2011 eConference package contains 65 talks, grouped in following topics: Single-Cell qPCR, Next Generation Sequencing, High throughput qPCR, MIQE guidelines, RNAi-miRNA, qPCR Data analysis, Circulating Nucleic Acids, Pre-analytical Steps, New qPCR Applications in Molecular Diagnostics, and more ... ... qPCR 2011 Event now available NOW as eConfernce =3D> http://eConference.qP= CR2011.net You can watch an in-depth demo presentation of our seminars and talks right here =3D> http://eseminars.bioEPS.com/player/?action=3Ddemo ---------------------------------------------------------------------------= ----- MIQE_qPCR APP for iPhone, iPad and iPod - iOS Universal http://MIQE-press.gene-quantification.info Get help from a special team of experts in qPCR while on the move. MIQE - qPCR helps you in reviewing scientific works and checking your own experiments, when qPCR is involved. Check your project's compliance to MIQE in minutes, have all required references to hand, and follow qPCR events and news. http://itunes.apple.com/app/miqe-qpcr/id423650002?mt=3D8 ---------------------------------------------------------------------------= ----- Forward Please send the qPCR NEWS to further scientists and friends who are interested in qPCR ! Best regards, Michael W. Pfaffl responsible Editor of the Gene Quantification Pages http://www.gene-quantification.info ---------------------------------------------------------------------------= ----- If this newsletter is not displayed correctly by your email client, please use following link: http://qPCRnews.gene-quantification.info/ The qPCR NEWS and the Gene Quantification Pages are educational sites with the only purpose of facilitating access to qPCR related information on the internet. 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