※ 引述《EGFP (百分之六十九的幸福)》之銘言： : 小女子想請問 : "The reaction rate is linear provided an A340 of approximately 0.8 is not : exceeded during the five-minute time course." : 以上這句話中的0.8是指什麼呀? : "Under standard assay conditions and volumes at 22℃ ,the baseline drift : produced by this reaction causes a A340/min of approximately 0.003." : 以上這句話中的0.003是指什麼? : 不知道有沒有人可以幫我解答~感激不盡! : 還有沒有人可以提供詳細的測GST這個酵素的活性方法步驟?(找好久都只有大略的方法) Measurement of GST Activity by CDNB Assay 1.Combine the following solutions for each test tube. 2.Votex the test tube to mix. Note: Due to its low solubility in water, CDNB may cause the solution to become slightly cloudy. The solution should clear upon mixing. 3.Transfer the above solution into UV-transparent cuvettes which had been added 50 l GST enzyme (Note: the blank tube without enzyme). 4.Cover each cuvette with wax film and invert to mix (optional). 5.Place these cuvettes in a UV spectrophotometer blank at 340nm. Measure the absorbance of the sample cuvette at 340nm and simultaneously start a timer. 6.Record the absorbance readings at 340nm at one-minute intervals for 5 minutes. Note: The reaction rate is linear provided an A340 of approximately 0.8 is not exceeded during the five-minute time course. Initially, results may be plotted to verify that the reaction rate is linear over the time course. Adjust the amount of sample containing the GST enzyme to maintain a linear reaction rate. 7.Calculate the A340/min/ml sample(see "Calculations"). 還是 你要 Western blotting 的? : 以請吃飯 看電影 或介紹男女朋友等之類的方法作為回報~ --

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