作者jvn (forever momory)
站内Biotech
标题[问题] 不好意思 请问一下PCR的doubling
时间Wed Aug 2 10:59:44 2006
我是生科领域的新手(大四毕业)
我想要做gene的mutagenesis
利用PCR的方法
但是我刚刚看current protocols
The average number of mutations per template increases as a function of
the number of doublings in the EP-PCR reaction (Table 8.3.1).
It should be noted that it is the number of doublings that is the determining
factor, rather than the number of EP-PCR cycles.
我想请问一下 PCR doubling既然不是cycle的话 到底是什麽呢 感谢唷
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