POD 的活性藉由测量维他命C在H2O2的氧化程度。 加入 50μl 悬浮物到950 μl potassium phosphate buffer (pH 7.0, 50 mM)（含0.1 mM EDTA、 0.5 mM ascorbate、and 0.1 mM H2O2。）後，维他命C开始氧化。反应30秒後 ，以分光光度计在波长290 nm时测量到吸收减少。在20°C进行分析。 One unit of POD was defined as the variance of absorbance per 107 cells (ΔA470/107 cells)。 问题一 请问维他命C氧化程度愈高 是否表POD愈没用？ 二 维他命C氧化程度愈高 颜色会愈浅 吸收愈少吗？ 测量SOD 的活性， the reaction buffer (700μl) was prepared with 50 mM potassium phosphate buffer (pH 7.8), 0.1 mM EDTA, 18 μM cytochrome c, and 0.1 mM xanthine. 加黄飘呤氧化脢（Xanthine oxidase） 到reaction buffer to get an increase of absorbance at 550 nm of ca. 0.025 min －1 at 25°C. Then, 15 to 100μl of supernatant sample and H2O was added to the reaction buffer, including xanthine oxidase, to give a volume of 800 μl. The rate of cytochrome c was measured at 550 nm for an additional minute. One unit of SOD was defined as the variance of absorbance per min per 107 cells ( A550/min 107 cells)。 问题三 请问SOD Xanthine oxidase cytochrome c 这三者间关系为何阿？？ --

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