作者singlewolf (application odyssey)
看板Biotech
标题[问题] 关於trypan blue-labeled albumin
时间Thu Nov 23 23:41:44 2006
请问有没有人知道如何将trypan blue接到BSA上呢
目前看到paper里面methods and materials提到
"Trypan blue labeled bovine serum albumin (TB-BSA) was prepared by adding
180mg Trypan blue (Sigma) and 4g BSA fraction V (Sigma) to 100mL HBSS (Gibco).
Precipitation of TB-BSA stock with 5% trichloracetic acide (TCA) showed that
greater than 98% of the Trypan blue was protein-bound. TCA precipitation
was also carried out on the TB-BSA after incubation with the test agent to
ensure that no cleavage of the dye from the protein was occurred."
看完之後还是不太了解该怎麽制备TB-BSA
请问有前辈可以提供相关protocol吗
又
是否有厂商有在卖TB-BSA?
谢谢 <(_ _)>
--
※ 发信站: 批踢踢实业坊(ptt.cc)
◆ From: 59.121.207.243