不知道在这里可不可以问这些问题 这是我第一次读paper 然後要报告 小弟程度真的不是很好 遇到很多问题 不知道有没有大大可以帮我解答 感机不尽>"< 篇名是difution characterist of collagen film <原文>Collagen films prepared by treating collagen gel solutions with different concentrations of glutaraldehyde were evaluated as a biodegradable and biocompatible drug carrier for cosmetically effective agents in this study. 在这个论文里论述胶原蛋白胶体溶液再不同浓度的 戊二酸醛里 讨论药物传输的生物降解性与生物相容 性(这一段是摘要的 cosmetically这个字真的就不知道 怎麽翻) Then the gel solutions were put into a jacketed container to measure the viscosity. A circulating water bath was connected to this jacketed container for maintaining the temperature of the container at 258C. *jacketed container 是什麽样的容器 *circulating water bath是什麽维持温度的系统!? 水蒸气吗? <原文> a suitable spindle was mounted and activated with a desired rotating speed,and measurements were taken after the designated temperature was reached and a stable reading was recorded. The viscosity was recorded at predetermined time intervals. An average of three replicates was reported for each time point. *spindle这是什麽形状 实验步骤有点看不懂 是先把它转成薄膜在量他的 黏度吗? <原文>A collagen solution at 1% (w/w) was prepared by dissolving collagen in 3% acetic acid. Then various concentrations of glutaraldehyde (0, 0.05, 0.075, 0.1, 0.2, 0.25, and 0.3%, v/w) were added, and these mixtures were immediately transferred into a round acrylic mold, which had been sealed with parafilm paper (American Can, USA) around the bottom.These molds were then placed in an electric dryer(Intech, Osaka, Japan) until the weight of the collagen film approached a constant value. Then, the parafilm paper was carefully peeled off, and the collagen films were stored in a desiccator at ambient temperature until use. The thickness of the collagen films was measured at five different randomly selected places. The uniformity of the thickness of collagen films was acceptable since the coefficient of variation was less than 5%. round acrylic mold是类似培养皿的样子吗? <翻译>把胶原蛋白溶在3%的醋酸溶液里配成1%的 胶原蛋白溶液 并加入(0 0.05 0.075 0.1 0.2 0.25 0.3v/w) 的戊二酸醛 并直接加入圆形丙烯酸的模子里 并用石蜡纸密封 并放置在电子乾燥器里 直到胶原蛋白膜的重量几乎不变 在把时蜡纸小 心的撕掉 并把胶原蛋白储藏在乾燥温度的环境 将厚的胶原蛋白膜在随机不同的地方量测 The uniformity of the thickness of collagen films was acceptable since the coefficient of variation was less than 5%. 这句就不大懂 <原文> Diffusion studies were carried out using retinoic acid (0.01%), retinol palmitate (0.1%), ascorbic acid 6-palmitate (0.1%), and tocopherol acetate (0.1%) as model drugs either in solution or a gel form (using1%, w/w, collagen as a gelling agent and 10%Cremophor RH40 as a solubilizer) at 378C, in a flow-through diffusion system. This system consists of a multi-channel peristaltic pump (202U/AA, Watson Marlow), a fraction collector (Retriever IV,ISCO, USA), a circulating water bath, and six units of flow-through diffusion cells. The flow-through diffusion cells contain two side arms, which enable the conduction of receiver-cell media from a peristaltic pump to a fraction collector. The temperature was maintained at 378C by circulating constant temperature water through the outer jacket of the receiver cell. The surface area of the receiver cell opening was 1.77 cm , and the volumes for the donor and receiver compartments were 0.8 and 0.1ml, respectively. A 0.9% normal saline solution (containing 0.01% sodium azide and 10% Cremophor RH40) was used as the receiver cell medium. The receiver cell media were stirred at 450 rpm by externally driven, teflon-coated magnetic bars. Collagen films prepared with different concentrations of glutaraldehyde were mounted onto each receiver cell, and an O-ring and cell top were placed on the top of each membrane. These com- ponents were then clamped securely in place. The receiver cell medium reservoir was maintained at 378C. Subsequently, a solution or gel form of the model drugs was applied on the top of each collagen film. All samples were collected over 10-h periods. The diffusion of model drugs through collagen films was followed by determining their concentrations in the collected medium as a function of time. The concentrations of these model drugs were analyzed by a validated HPLC method as described below. 1.multi-channel peristaltic pump (202U/AA,Watson Marlow), a fraction collector (Retriever IV,ISCO, USA), a circulating water bath (这些是怎样的仪器!?) 2.因为很多仪器都没有使用过 所以真的不懂他的 实验流程 可能要麻烦大大解说一下 不用翻译 大概说明一下他的实验流程 好长一篇 怕没有人回 很快就要报告了 真的需要大家帮我 也希望给我一些意见 对於以後做专题 还有读paper 感谢各位大大 --

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