以下是我在网路上找到的方法 我有用过是可以的 有兴趣没钱买kit的可以试试看 4x10(6次方) target cells collect the cell sample in 1.5 ml eppendorf tube --> spin down --> resuspend with 0.5 ml PBS in 1.5 ml eppendorf tubes 55ul of lysis buffer (40 ml of 0.5 M EDTA / 5 ml of 1 M Tris-Cl buffer pH 8.0 / 5 ml of 100% Triton X-100 / 50 ml of H2O) --> 4℃ 20min --> 4℃ 12,000g 30 min --> extract the supernatant with 1:1 mixture of phenol:chloroform --> gentle agitation for 5 min --> 4℃ 12,000g 5min --> 2V cold Ethanol+ 1/10V NaoAc --> 4℃ 12,000g 5min --> 30ul of deionized water-RNase solution --> 30 minutes at 37℃ --> Run the 1.2% gel at 5V for 5min before increasing to 100V 来源 http://hedricklab.ucsd.edu/Protocol/DNAFRAG.html (Hedrick Lab, UCSD Cancer Center) --

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