作者sdshow (蝶)
看板Biotech
标题Re: [求救] 请问virus infection时加入的polybrene
时间Thu Jan 17 11:33:04 2008
http://pingu.salk.edu/~sefton/Hyper_protocols/infectRetro.html
这边有提到一些~~~~
Infection with retroviruses is facilitated greatly by polybrene.
This is a small, positively charged molecule that binds to cell
surfaces and neutralizes surface charge.
This apparently allows the viral glycoproteins to bind more
efficiently to their receptors, because it reduces the repulsion
between sialic acid-containing molecules.
To use polybrene optimally, the cells to be infected should
be pre-treated with polybrene and the virus adsorption should
be done in the presence of polybrene.
※ 引述《momyourb (不死无敌小强)》之铭言:
: 请问各位,我在virus infection的protocol上看到
: 需要加入polybrene,上网查了一下大概是可以增加
: 病毒颗粒对细胞的表面结合能力,但是其机制是什麽啊?
: 有更详细的说明吗?
: 另外请问一下polybrene哪家有在卖啊?
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