看板Biotech
标 题Re: [问题]pET28a的表现效率
发信站批踢踢参 (Fri Mar 14 03:30:11 2008)
转信站ptt!Group.NCTU!grouppost!Group.NCTU!ptt3
Hi,
I have no experience in expression of protein lower than 10kD either.
But I would think pET28 is an ok system, since smaller protein is
generally easier to express. I would give it a try. Good luck!
※ 引述《[email protected] (看山 望海 吹风)》之铭言：
: 请教一下，pET28a可以大量表现10 kDa以下的蛋白质吗？
: 因为我之前常用来表现的蛋白都在20 kDa以上。
: 这次找到的目标蛋白却只有84个a.a.，我担心pET28a没办法做出来。
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※ 发信站: 批踢踢参(ptt3.cc)
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