Hi, Thanks for your question. To be honest with you, that was basically my personal experience, and I have no standard answer to it. :) I agree with the point you brought up, that it might actually take less energy for the cellular trancription/translation machineries to carry out a complete cycle of transcription/translation of a smaller gene. My explanation is, there is less chance for the premature termination of transcription/translation to ocurr since the gene is smaller. For instance, longer genes might have more "rare codons" for the host bacteria (bacteria have less corresponding tRNAs for these codons. One example is the arginine codon AGA) It is believed that the rare codons cause pausing of translation in bacteria. Therefore it takes additional time/energy to complete a translation cycle for a longer gene. ※ 引述《Ren (wisc)》之铭言： : ※ 引述《[email protected] (Janeliu)》之铭言： : : Hi, : : I have no experience in expression of protein lower than 10kD either. : : But I would think pET28 is an ok system, since smaller protein is : : generally easier to express. I would give it a try. Good luck! : Could you please explain why it's "generally easier" to express? : Simply because it's smaller (which may require less energy)? --

※ 发信站: 批踢踢实业坊(ptt.cc) ◆ From: 68.197.145.28 ※ 编辑: Janeliuu 来自: 68.197.145.28 (03/14 09:13) ※ 编辑: Janeliuu 来自: 68.197.145.28 (03/14 09:22) ※ 编辑: Janeliuu 来自: 68.197.145.28 (03/14 09:23)