作者inferno6562 (地狱泡面)
看板Biotech
标题[求救] cell lysis buffer成分的用处
时间Sun Mar 16 23:44:28 2008
Cells were lysed either in Triton cell lysis buffer (10mM Tris, pH 7.05,30 mM
NaPPi, 50mM NaCl, 1% Triton X-100, 2mM Na3VO4, 50mM NaF, 20mM
β-glycerophsphate and freshly added 0.5 mM PMSF, 0.5 μg/ml Leupeptin,
0.5 μg/ml pepstatin, 1μg/ml microcystin)
想知道夸号内成分在lysis buffer的用处
谢谢
我是代po的 如果有什麽专有名词打错了请见谅>"<
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1F：推 jsi:我觉得这问题应该要自己先去查资料 查不到再来问吧 03/17 01:06
2F：推 allisa:Na3VO4跟PMSF都是proteinase inhibitor 03/17 12:14
3F：推 yowsien:Na3V04 NaF 是Phosphatase inhibitor 03/17 21:55