最近看的发炎相关paper 其中adipocyte和macrophage做co-culture实验的部分 一直不太懂 希望版上的各位能够帮忙> < 以下为原文 The co-culture of adipocytes andmacrophages was performed in two different ways as follows. In the contact system, the differentiated 3T3-L1 and RAW 264.7 macrophages were independently preincubated with 1,25-(OH)2D3 for 24 h. Then, the macrophages were plated onto a 3T3-L1 monolayer, incubated with TNF-alpha(15 ng/mL) for 3 h, and harvested. In the transwell system, the two types of cells were independently preincubated with 1,25-(OH)2D3 for 24 h. Then, the cells were co-cultured using transwell inserts with a 0.4 μm porous membrane (Dutscher, Issy-les-moulineaux) to separate the adipocytes from the macrophages. After incubating with TNF-alpha(15 ng/mL) for 3 h, the cells in the lower well (adipocytes) were harvested. 问题一：为何要做contact和transewell两种不同的方式? 问题二：transewell system就是transwell assay吗? --

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