作者love30118x (挤抠)
看板Biotech
标题[求救] 新手求步骤
时间Fri Oct 30 22:13:57 2015
Construction of the pPre-dsRBD plasmid:
Vector: pBase digested with Pst I and Bgl II, and gel-purify 2.7 kb
Insert: pCR-dsRBD digested with Pst I/Bgl II, and gel-purify 227 bp
dsRBD of pET47b-dsRBD plasmid (kindly provided by Scott Showalter, PLoS One
7:e51829, 2012) was PCR-amplified by dsRBD-U1 and dsRBD-D1 primers, then
cloned into pCR T7/CT TOPO named pCR-dsRBD
dsRBD-U1 (39mer): 5'-AACTGCAGACCGCGTAGTCCGGTTCGTGAACTGCTGGAA-3' (5'-terminus
has a Pst I site)
dsRBD-D1 (39mer): 5'-TTAGATCTTGCTATTCGGAACCTGCGGCTGATTTGCTTT-3' (5'-terminus
has a Bgl II site)
最近要做这个
已经先做好了一罐
pCR-dsRBD 0.062ul
和
pBase 0.128 ul
但是毫无头绪再来要做什麽
救命~
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1F：→ blence: 0.062ul这麽少的体积你用什麽取跟定量阿 10/30 23:04
2F：→ blence: 是大学生的实验课？ 10/30 23:04
3F：→ turtle24: 要做的事是不是已经写在前三行了 =w=? 10/31 00:00
4F：→ Ice9: google "current protocols in molecular biology" 10/31 11:32
5F：推 ABULA666: 这个问你身边的学长姐比较快得到答案吧?? 10/31 14:12