作者x30621 (1232131213)
看板Biotech
标题[求救] 请问蒸馏水 算是一次水(RO水)吗!
时间Wed Nov 30 22:00:45 2016
小弟要染IHC ,其中DAB染色液 说明书说不能用去离子水,要用蒸馏水
NOTES:
We recommend using glass-distilled water in the preparation of substrate buff
er. Deionized water may con-
tain inhibitors of the peroxidase reaction. Solutions containing sodium azid
e or other inhibitors of peroxidase
activity should not be used in diluting the peroxidase substrate.
想问各位用一次水(RO水)这样可以吗?
感谢各位大德…
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1F:推 abcam: 简单说 RO水~ 11/30 22:28
2F:推 susu8n0: 我用过生水染过,还不错的说~ 还可以再用铁汤匙搅一搅 11/30 22:59
3F:→ susu8n0: 提供铁离子~ 11/30 23:00
4F:→ x30621: 我之前用二次水 阳性 非常淡的说 11/30 23:38
5F:→ x30621: 才发现可能是要用蒸馏水的样子 11/30 23:40
6F:推 airrooco: Deionized water may contain inhibitors....很好奇是 12/01 07:31
7F:→ airrooco: 什麽东西... 12/01 07:32
8F:推 aggaci: 蒸馏水是HPW High purity water跟RO水完全不一样 12/03 23:12