课程名称︰分析化学丁 课程性质︰必修 课程教师：张焕宗 开课学院： 开课系所︰ 考试日期（年月日）︰2009/5/18 考试时限（分钟）：150min 是否需发放奖励金：是 谢谢 （如未明确表示，则不予发放） 试题 : I.Differentiate the two terms of each pair(4pts each;48 pts total) 1.displacement titration and indirect titration 2.masking agent and auxiliary complexing agent 3.standard reduction potential and formal reduction potential 4.coulometry and electrogracimetric analysis 5.amperometry and voltammetry 6.faradic current and charging current 7.sample current polarography and square wave voltammetry 8.fluorescence and phosphorescence 9.isobestic point and isoelectric point 10.diffusion current and residual current 11.internal conversion and intersystem crossing 12.avsorbance and molar absorptivity II Fill in the blank(4pts each 52pts total) 1.Arrange the order(high-low) of the reduction potential for Pb2+/pb in:(A) 1M KCl (B) 1MKCl+0.5M EDTA (C) 1MKCl+O.1M EDTA (D)1M KCl+0.1M NH3 2.Arrange the order(high-low) of the formation constant for MY^n-4 that the metal ions are Fe2+,Fe3+, Ca2+,Na+ 3.______is a good masking agent for titrating Mg2+ with EDTA in the present of Ag+ 4.A Clark electrode is used to measure______ 5.Why is a mediater used in a sensor for the detection of glucose?______ How does the sensor work?_________________________________ 6.Using a nitrate(NO3-)electrode, nitrate standards of 0.005 and 0.01M give readings of-108.6 and-125.2 mV ,respectively.What is the concentration og nitrate(solution A) to give a reading of -199.6mV? 7._______ is technique that allow concentration and detection of 0.1nM Pb2+ 8.If E0 values for AgCl/Ag and Ag+/Ag are 0.240 and 0.799V, respectively, the ksp for AgCl is______ 9.A current developing at the interface of two solutions is called_____ 10.If an electrode has a selectively cofficient kF- OH-=0.1, the electrode potential in a solution of 0.01mM F- at pH10.0 is equal to a constant +__mV 11.Give an example of metal ion buffer: 12.Describe the Beer's law: III. Consider the titration of 100.0ml of 2.0mM Zn2+ with 2.0 mM EDTA at pH10 in the presence of 0.1 M NH3(Kf for ZnY^2- =10^16.50) (A) write down the equation to express the relationship between alphaY4- and H+ (B) write down the structure of EDTA. How many moles of EDTA do complex with one mole Zn2+ (C) Find pZn2+ after addition of 10,000 and 110ml EDTA(alphaY4-=0.36) IV. A solution contains 0.100 M Ce 3+, 1.00mM Ce4+, 1.00mM Mn2+, 0.100M Mn04- and 1.00M HClO4(E0 for Ce4+/Ce3+= 1.72 V,E0 for Mn04/Mn2+=1.507) (A) Calculate E for the conditions given. (B) At what pH would the given concentratiom of Ce4+,Ce3+,Mn2+,and Mn04- be in equilibrium at 298K (C) Draw a scheme of the cell (D) Use a line notation to wxpress the cell V. Ascorbic acid(0.01M) was added to 10.0ml of 0.0200M Fe2+ in a solution buffered to pH3.0. E0 values for dehydroascorbic acid /ascorbic acid and Fe3+/Fe2+ are 0.390 and 0.767V ,respectively. Calculate the cell voltage when(A)5.0 (B) 10.0 (C) 15.0 ml of ascorbic acid have been added. VI. Expain the principle of operation of a liquid-base ion selective electrode VII. Immunoassay (A) Describe ELISA (B) Why is an enzyme used in ELISA (C) How can TNT be determined by conducting an immunoassay? VIII Describe an optical method to determine the pKa of an acid IX Absorption and flurscence (A) Write down the relationship between the singal and analyte concentration (B) Use scheme to show the difference between a spctrophotometer and a fluorometer ( you have to write down the name of components) (C) Compare absorption and florescence in terms od sensitivity and selectivity --

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